Effects of D2O on the association-dissociation equilibrium in subunit proteins.

Effects of D , O on the Association - Dissociation Equilibrium in Subunit Proteins

Heavy water has been used as a tool to study the role of water in the association-dissociation equilibrium of subunit proteins. In the case of lactate dehydrogenase, DzO was found to stabilize the enzyme at low ionic strength and inhibit subunit interchange between heartand muscle-type enzyme in solution. When frozen, however, subunit interchange occurred to a similar extent in either DzO or Hz...

tudy on sex steroid-binding proteins (with emphasize on 17 -estradiol) in plasma of female and juvenile kutum (Rutilus frisii kutum)

A sex steroid-binding protein (SBP) that binds to 17 b-estradiol with high affinity and moderate capacity was identified in the plasma of female and juvenile of kutum (Rutilus frisii kutum) sampled during the early stage of gonadal maturation in October and prior to spawning in March. Mean maximum specific binding (Bmax) and equilibrium dissociation constant (Kd) of the fish were as follows: In...

Three-Body Dissociation Dynamics of Excited States of O3(D2O)

In these experiments, the dynamics of the three-body dissociative photodetachment of O3(D2O) at 258 nm are directly probed for the first time. Photodetachment of a negatively charged precursor, with coincident energy analysis of the photoelectron, allows production of energy-selected excited-state O3(D2O) complexes. By measurement of the laboratory velocities and recoil angles of the O + O2 + D...

Numerical Study of Non - Equilibrium Air Dissociation For Calculation of Electron Density in Hypersonic Flow

Recombinant Production of a Novel Fusion Protein: Listeriolysin O Fragment Fused to S1 Subunit Of Pertussis Toxin

Background: Some resources have suggested that genetically inactivated pertussis toxoid (PTs) bear a more protective effect than chemically inactivated products. This study aimed to produce new version of PT, by cloning an inactive pertussis toxin S1 subunit (PTS1) in a fusion form with N-terminal half of the listeriolysin O (LLO) pore-forming toxin. Methods: Deposited pdb structure file of the...